CIMA™ – Confocal Hyperspectral Microscope

CIMA is a hyperspectral confocal system sensitive between 400 nm and 1700 nm. Spectral resolution can be as low as 0,2 nm in the visible range, and 0,6 nm in the infrared. It pairs a galvanometer head and one of the fastest and most sensitive cameras on the market to yield an acquisition rate above 300 spectra per second. CIMA provides three acquisition modes: confocal hyperspectral imaging, multispectral fluorescence imaging, and emission spectroscopy of a sample in a cuvette.

CIMA Applications Overview

• Simultaneously study the spatial distribution and spectral properties of complex nanomaterials such as upconverting nanoparticles and quantum dots.
• Perform in vitro or in vivo high resolution hyperspectral microscopy.
• Develop new sensors based on environment dependent spectral shifting of their fluorescence signal.

Technical Specifications

VIs

IR

Spectral range

400 nm – 1700 nm

400 nm – 1700 nm

Spectral resolution (FWHM)

< 0.2 nm (Custom resolution available upon request)

< 0.6 nm (Custom resolution available upon request)

Spatial resolution

Diffraction Limited

Diffraction Limited

Cameras

Back-illuminated CCD or EMCCD

InGaAs linear array

Excitation wavelength

980 nm (other wavelengths available). 3 laser input ports. UHP mercury lamp 130 W.

980 nm (other wavelengths available). 3 laser input ports. UHP mercury lamp 130 W.

Microscope

Scientific grade, inverted.

Scientific grade, inverted

Confocal Mode

VIS

IR

Scanning region

300 µm x 300 µm (20X objective), 100 µm x 100 µm (60X objective)

300 µm x 300 µm (20X objective), 100 µm x 100 µm (60X objective)

Pinhole diameter

30 µm to 200 µm

30 µm to 200 µm

Cuvette Mode

VIS

IR

Cuvette size

Standard 10 mm x 10 mm (3.5 ml)

Standard 10 mm x 10 mm (3.5 ml)

Fluorescence Mode

VIS

IR

Camera

Megapixel colour camera for fluorescence and sample visualization

Megapixel colour camera for fluorescence and sample visualization

Illumination lamp

UHP mercury lamp 130 W

UHP mercury lamp 130 W

Epifluorescence filter cubes

Up to 6 different filter cubes

Up to 6 different filter cubes

Publications

• Journal of Visualized Experiments / Life Science: 
  Hyperspectral Imaging as a Tool to Study Optical Anisotropy in Lanthanide-Based Molecular Single Crystals
• Inorganic Chemistry / Life Science
  Microwave-Assisted Solvothermal Synthesis of Upconverting and Downshifting Rare-Earth-Doped LiYF4 Microparticles
• Chemistry a European Journal / Life Science
  Probing Optical Anisotropy and Polymorph-Dependent Photoluminescence in [Ln2] Complexes by Hyperspectral Imaging on Single Crystals

Custom products